| 质粒名称: | pSJS 1240 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12234 |
| 存储实验室: | Sung-Hou Kim |
|
载体骨架基本信息 |
|
| 载体名称: | pSJS1240 |
| 载体出品公司: | -- |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 4245 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSJS 1240 was a gift from Sung-Hou Kim (Addgene plasmid # 12234 ; http://n2t.net/addgene:12234 ; RRID:Addgene_12234) |
| 参考文献部分: | Overexpression of archaeal proteins in Escherichia coli. Rosalind Kim, Steve J. Sandler, Stanley Goldman, Hisao Yokota, Alvin J. Clark and Sung-Hou Kim. Biotechnology Letters. March 1998. 20(3): 207-210. 10.1023/A:1005305330517 |
买家导航
pSJS1240载体基本信息
pSJS1240载体图谱质粒图谱和多克隆位点信息
pSJS1240载体简介
pSJS1240载体序列
LOCUS pSJS 1240 5614 bp ds-DNA circular SYN 19-SEP-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSJS 1240
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5614)
AUTHORS Rosalind Kim, Steve J. Sandler, Stanley Goldman, Hisao Yokota, Alvin
J. Clark, Sung-Hou Kim
TITLE Overexpression of archaeal proteins in Escherichia coli
JOURNAL Biotechnology Letters. March 1998. 20(3): 207-210.
REFERENCE 2 (bases 1 to 5614)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5614
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1..546
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 447..466
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 663..680
/label=L4440
/note="L4440 vector, forward primer"
promoter 1072..1174
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 1175..1834
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(1241..1260)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS complement(3673..4461)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
tRNA complement(4981..5057)
/gene="argU"
/product="E. coli tRNA-Arg recognizing AGA and AGG"
/label=argU
tRNA 5304..5379
/gene="ileX"
/product="E. coli tRNA-Ile recognizing AUA"
/label=ileX
ORIGIN
1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
1861 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
1921 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc
1981 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
2041 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt
2101 tattctgcct cccagagcct gataaaaacg gttagcgctt cgttaataca gatgtaggtg
2161 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
2221 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgctgccg
2281 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
2341 tgccccgcgc ccaccggaag gagctaccgg cagcggtgcg gactgttgta actcagaata
2401 agaaatgagg ccgctcatgg cgttgactct cagtcatagt atcgtggtat caccggttgg
2461 ttccactctc tgttgcgggc aacttcagca gcacgtaggg gacttccgcg tttccagact
2521 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
2581 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
2641 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
2701 ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
2761 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
2821 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
2881 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
2941 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
3001 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
3061 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
3121 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
3181 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
3241 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
3301 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
3361 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
3421 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
3481 ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
3541 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcgagctcga cccgcatagt
3601 cttccccagc tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg
3661 aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa
3721 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat aagcctgcct
3781 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
3841 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
3901 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
3961 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
4021 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
4081 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
4141 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
4201 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
4261 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
4321 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
4381 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
4441 ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc gcgaagcggt
4501 gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt accagtacat
4561 cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa tgccgccgag
4621 agtaaagcca cattttgcgt acaaattgca ggcaggtaca ttgttcgttt gtgtctctaa
4681 tcgtatgcca aggagctgtc tgcttagtgc ccactttttc gcaaattcga tgagactgtg
4741 cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag aggctagatc
4801 gttccatgtt gagttgagtt caatcttccc gacaagctct tggtcgatga atgcgccata
4861 gcaagcaggt cgagctcgca tgcgaggaaa atagacgaca tttttggtga taatgtccca
4921 aatatgtccc actctgaaat tatggaggat ataaagaagg cgtaactgat tgaattgtaa
4981 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
5041 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
5101 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
5161 ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg tctgaatcaa
5221 gaaaacccgt attttcatgt atcaaagtga cctgcagcca agcttggatt gcgacacgga
5281 gttactttat aatccaatcg attggcccct tagctcagtg gttagagcag gcgactcata
5341 atcgcttggt cgctggttca agtccagcag gggccaccag cggccgcaaa ggctgacgag
5401 aaatcgtcag cctttttaag ctttaagccg aattccagca cactggcggc cgttactagt
5461 ggatccgagc tcggtaccaa gcttatcgat gataagctgt caaacatgag aattacaact
5521 tatatcgtat ggggctgact tcaggtgcta catttgaaga gataaattgc actgaaatct
5581 agaaatattt tatctgatta ataagatgat cttc
//
